First report of natural infection by Trypanosoma cruzi in secretions of the scent glands and myocardium of Philander opossum (Marsupialia: Didelphidae): Parasitological and clinicopathological findings.

Trypanosoma cruzi is the etiologic agent of American trypanosomiasis and can infect humans and different species of domestic and wild animals. The marsupials are important wild reservoirs of T. cruzi, aiding in the maintenance of this agent in sylvatic and peri-domestic environments. The objective of this study was to report the parasitological and clinicopathological findings of a natural infection by T. cruzi in one specimen of Philander opossum that originated from the Brazilian Amazon. The animal was captured in a forest fragment near a rural community with reports of human Chagas disease. T. cruzi infection was diagnosed by blood smear examinations, blood culture, scent glands secretion culture, histopathological examination, and nested-PCR. Positive samples were subjected to PCR to characterize the discrete typing units (DTUs) of T. cruzi. Characteristic trypomastigotes of T. cruzi were observed in the blood smear, and spheromastigotes, epimastigotes, and trypomastigotes were visualized in the cultures. Non-suppurative myocarditis associated with amastigote clusters was the principal histopathological finding. DNA from T. cruzi was detected in samples of blood, blood cultures, scent glands secretion cultures, cardiac muscles, and the spleen. The TcI and the TcII/V/VI group DTUs were detected in blood culture and scent glands secretion cultures. Infection by T. cruzi can cause myocarditis in P. opossum and DTUs TcI and TcII/V/VI group mixed infection can be detected in the acute phase. P. opossum can be a source of infection for triatomine vectors and has the potential source for direct transmission of T. cruzi by secretions from the scent glands. These data are important to improve the understanding of the complex enzootic transmission cycle of T. cruzi in the Brazilian Amazon.

Plasmodium vivax and Plasmodium falciparum infection in Neotropical primates in the western Amazon, Brazil.

The Brazilian Amazon is endemic for malaria and natural infections by Plasmodium spp. have been detected in Neotropical primates. Despite the diversity of primate species in the region, studies on infections by these agents are limited. The aim of the present study was to investigate the frequency of infection by Plasmodium vivax and P. falciparum in free-born primates that were kept in captivity, in the western Amazon, Brazil. Blood samples were collected from 98 Neotropical primates. Detection of P. vivax and P. falciparum DNA was performed using a semi-nested PCR, and the amplified products were sequenced. Plasmodium spp. DNA was detected in 6.12% (6/98) of the primates. P. vivax, and P. falciparum DNA was detected in 2.04% (2/98) and 4.08% (4/98) of these mammals, respectively. Sequencing and phylogenetic analysis confirmed the results obtained from the semi-nested PCR. The presence of infected non-human primates (NHP) can be auxiliary in the maintenance of P. falciparum and P. vivax and may have implications for the malaria surveillance and control in the Brazilian Amazon. It is necessary to structure an efficient surveillance system for the aetiological agents of malaria that infect NHP and humans to reduce the risk of Plasmodium spp. introduction into new areas, to protect all susceptible species.

Dirofilaria immitis infection in dogs in Algodoal Island, Brazilian Amazon / Infecção por Dirofilaria immitis em cães na Ilha de Algodoal, Amazônia brasileira

Dirofilaria immitis, a parasite that mainly infects domestic or wild canids, but can infect felines or humans as well, is frequent in many Brazilian areas. The main objective of this research was to determine the prevalence of natural canine infection at the Algodoal-Maiandeua Island complex, in the coastal region of the state of Pará, Brazil. A total of 67 dogs were sampled for blood microfilariae detection and for D. immitis DNA detection. Microfilaria and D. immitis DNA could be detected in 35.8% (24/67) of the animals. In one dog's sample no microfilariae were detected, but the PCR was positive, suggesting that either larvae recently were eliminated or adults died shortly before sample collecting. Therefore, it can be concluded that the occurrence of D. immitis is a health threat for domestic and wild canids at the Island of Algodoal, as well as for feline or human health.(AU)

Dirofilaria immitis, um parasito que infecta principalmente canídeos domésticos ou selvagens, embora também possa infectar felinos e humanos, é frequente em muitas áreas do Brasil. O objetivo deste estudo foi determinar a ocorrência da infecção natural em cães provenientes do complexo da Ilha de Algodoal-Maiandeua, região litorânea do estado do Pará, Brasil. Um total de 67 cães tiveram o sangue coletado para detecção de microfilárias de D. immitis e seu DNA. Microfilárias e o DNA de D. immitis foram detectados em 35,8% (24/67) dos animais. Na amostra de um animal, não foram observadas microfilárias, mas o seu DNA foi detectado, sugerindo que as larvas tenham sido recentemente eliminadas ou os adultos tenham morrido antes da coleta da amostra. Portanto, pode-se concluir que a ocorrência de D. immitis é uma ameaça à saúde de canídeos domésticos no complexo da Ilha de Algodoal-Maiandeua, bem como para felinos e seres humanos.(AU)

Trypanosoma cruzi infection in captive Neotropical primates in the Brazilian Amazon.

The aim of this study was to detect the infection by Trypanosoma cruzi in captive Neotropical primates in the Brazilian Amazon. From February 2013 to July 2014, 112 blood samples were collected from Neotropical primates from the Amazonas, Amapá, and Pará States, north of Brazil. The subjects belonged to the families Cebidae (N = 59), Atelidae (N = 41), Callitrichidae (N = 5), Pitheciidae (N = 4), and Aotidae (N = 3). Blood smears also were examined for the presence of trypomastigotes by optical microscopy. For the detection of T. cruzi DNA, a Nested-PCR with primers TCZ1/TCZ2 and TCZ3/TCZ4 was performed. T. cruzi DNA was detected in 12.5% (14/112) of Neotropical primates examined. Positive samples were detected in 16%, 12.5%, and 11.11% of the different species of primates sampled from the Amapá, Pará, and Amazonas states, respectively. The analysis of the blood smears did not reveal trypomastigote forms of T. cruzi. In conclusion, Neotropical primates kept in captivity were infected by T. cruzi in the studied areas. We recommend that a health management protocol be put into place to prevent the transmission of infectious agents among captive populations, captive and wild populations, and between NHPs and the technicians who handle these animals.

Leishmania infantum infection in dogs from maroon communities in the Eastern Amazon / Infecção por Leishmania infantum em cães de comunidades quilombolas na Amazônia Oriental

ABSTRACT: This study was designed to detect L. infantum infection in dogs and to evaluate the factors associated with canine visceral leishmaniasis in the maroon communities of Menino Jesus de Petimandeua and Itaboca in the municipality of Inhangapi, Pará, Brazil. Whole blood and intact skin samples were collected from 143 dogs, and a questionnaire was applied. L. infantum DNA was detected by polymerase chain reaction (PCR) using primers RV1 and RV2. Collection sites were georeferenced to obtain a spatial distribution of the residences visited and infected dogs. L. infantum DNA was detected in 8.4% (12/143) of the skin samples and in 1.4% (2/143) of the blood samples. On the risk map, three clusters were observed in Itaboca and one was observed in Menino Jesus de Petimandeua. We observed that most of the inhabitants in these maroon communities live close to forested areas and do not use protection against insect vectors. The presence of canine reservoirs of L. infantum associated to environment characteristics (preserved forests and deforested areas) and habits of dog owners (living near forested areas and not using any protection against insects) may favor the transmission of L. infantum in the studied areas.

RESUMO: O presente estudo objetivou detectar a infecção por Leishmania infantum em cães e avaliar os fatores associados com a leishmaniose visceral canina nas comunidades quilombolas Menino Jesus de Petimandeua e Itaboca, município de Inhangapi, Pará, Brasil. Foram coletadas amostras de sangue e de pele íntegra de 143 cães, sendo aplicado um questionário. A detecção do DNA de L. infantum foi realizada através da PCR com os iniciadores RV1 e RV2. Os locais de coleta foram georreferenciados para realizar a distribuição e análise espacial das residências visitadas e dos cães infectados. DNA de L. infantum foi detectado em 8,4% (12/143) das amostras de pele dos cães e em 1,4% (2/143) das amostras de sangue. No mapa de risco, foram observados três aglomerados em Itaboca e um em Menino Jesus de Petimandeua. Pôde-se constatar que a maioria dos moradores das comunidades quilombolas reside em áreas próximas de mata e não utilizam proteção contra insetos vetores. A presença de reservatórios caninos de L. infantum associada com características ambientais (floresta preservada e áreas de desmatamento) e os hábitos dos proprietários dos cães (que vivem perto de áreas de floresta e não usam qualquer tipo de proteção contra insetos) podem favorecer a transmissão de L. infantum nas áreas estudadas.

Occurrence of eggs and oocysts of gastrointestinal parasites in passerine birds kept in captivity in Para State, Brazil / Ocorrência de ovos e oocistos de parasitos gastrointestinais em aves Passeriformes mantidas em cativeiro no estado do Pará, Brasil

ABSTRACT: The objective of this study was to detect helminth eggs and protozoan oocysts in samples of feces from birds of the order Passeriformes in Para State, Brazil. Fecal samples were collected individually from 403 passerine birds seized and kept in captivity in Para State. Samples were processed by the double centrifugation technique in saturated sucrose solution and the coccidial oocyst-positive samples were submitted to sporulation in potassium dichromate 2.0%. Helminth eggs and/or protozoan oocysts were observed in 43.18% (174/403) of the fecal samples examined. Coccidial oocysts were detected in 93.68% (163/174) of the positive samples, whereas helminth eggs were observed in 10.34% (18/174) of the positive samples. Oocyst sporulation occurred in 43.56% (71/163) of the samples, and only Isospora spp. oocysts were detected. Nematode eggs of the superfamilies Trichostrongyloidea (4.60%; 8/174), Ascaridoidea (0.57%; 1/174), and Trichuroidea (0.57%; 1/174) were diagnosed in the positive samples. Cestoda eggs were diagnosed in 2.87% (5/174), whereas Trematoda eggs were detected in 2.30% (4/174) of positive samples. Passerine birds seized and kept in captivity in the visited local presented parasitism by intestinal helminths and protozoan, with a predominance of infection with coccidia of the gender Isospora.

RESUMO: O objetivo do presente estudo foi detectar ovos de helmintos e oocistos de protozoários em amostras de fezes de aves da ordem Passeriformes no estado do Pará, Brasil. Amostras de fezes foram coletadas individualmente de 403 aves Passeriformes oriundas de apreensão e mantidas em cativeiro no estado do Pará. As amostras foram processadas usando a técnica de dupla centrifugação em solução saturada de sacarose e as amostras positivas para oocistos de coccídios foram submetidas à esporulação em dicromato de potássio 2,0%. Ovos de helmintos e/ou oocistos de protozoários foram observados em 43,18% (174/403) das amostras fecais examinadas. Oocistos de coccídios foram detectados em 93,68% (163/174) das amostras positivas, enquanto que ovos de helmintos foram observados em 10,34% (18/174). A esporulação de oocistos ocorreu em 43,56% (71/163) das amostras, e somente oocistos de Isospora spp. foram detectados. Ovos de nematódeos das Superfamílias Trichostrongyloidea (4,60%; 8/174), Ascaridoidea (0,57%; 1/174) e Trichuroidea (0,57%; 1/174) foram diagnosticados nas amostras positivas. Ovos de Cestoda foram diagnosticados em 2,87% (5/174), enquanto que ovos de Trematoda foram detectados em 2,30% (4/174) das amostras positivas. Aves Passeriformes oriundas de apreensão e mantidas em cativeiro nas áreas visitadas estavam parasitadas por helmintos e protozoários, predominando a infecção por coccídios do gênero Isospora.

F200Y polymorphism of the ß-tubulin isotype 1 gene in Haemonchus contortus and sheep flock management practices related to anthelmintic resistance in eastern Amazon.

The objective of the present study was to determine the frequency of the F200Y polymorphism in the ß-tubulin isotype 1 gene of Haemonchus contortus from various sheep flocks in eastern Amazon, and to identify management practices that may favor the emergence of resistance to anthelmintic drugs in the same area. In total, 305 specimens of H. contortus were collected from sheep at 12 farms located in the state of Pará. An allele-specific PCR was performed to detect the F200Y polymorphism, and questionnaires were used to obtain information about the farms and flocks. All genotypes were detected as follows: 31% of the parasites were RR, 37% of the parasites were SR, and 32% were SS. The completed questionnaires revealed that all farms employed semi-intensive farming systems, performed suppressive anthelmintic treatment, and based their choice of drug on cost and availability rather than on any knowledge regarding drugs that remained effective on their property. It can thus be concluded that the SNP in codon 200 of the ß-tubulin isotype 1 gene is present in the H. contortus populations from eastern Amazon, and that a series of management practices that favor the emergence of anthelmintic resistance are employed on these farms.

Molecular detection of Trypanosoma evansi (Kinetoplastida: Trypanosomatidae) in procyonids (Carnivora: Procyonidae) in Eastern Amazon, Brazil / Detecção molecular de Trypanosoma evansi (Kinetoplastida: Trypanosomatidae) em procionídeos (Carnivora: Procyonidae) na Amazônia Oriental, Brasil

ABSTRACT: The present study aimed to diagnose the natural infection of captive and free-living procyonids with Trypanosoma evansi in the states of Amapá and Pará, Brazil. From February 2012 to August 2013, whole blood samples and blood smears were obtained from 45 free-living procyonids and from nine procyonids kept in captivity in wild life refuges and zoobotanical parks in the states of Amapá and Pará. Whole blood samples were collected and kept at -20ºC for the detection of T. evansi DNA by PCR using the RoTat 1.2 forward and RoTat 1.2 reverse primers. In addition, the blood smears were processed and examined for the presence of trypomastigote forms of T. evansi. T. evansi DNA was detected in 18.52% (10/54) of the procyonids, namely, in captive crab-eating raccoons and captive and free-living coatis in Pará State. No trypomastigote forms were observed in the blood smears. DNA from T. evansi was detected in P. cancrivorus and N. nasua in Pará State, being this the first such report in P. cancrivorus.

RESUMO: O objetivo do presente trabalho foi realizar o diagnóstico da infecção natural por Trypanosoma evansi em procionídeos de vida livre e de cativeiro dos estados do Amapá e Pará, Brasil. Durante o período de fevereiro de 2012 a agosto de 2013, amostras de sangue total e esfregaços sanguíneos foram obtidos de 45 procionídeos de vida livre e de nove mantidos em cativeiro em mantenedores e Parques Zoobotânicos dos estados do Amapá e Pará. As amostras de sangue total foram coletadas e mantidas a -20ºC para pesquisa de DNA de T. evansi pela PCR utilizando-se os iniciadores RoTat 1.2 forward e RoTat 1.2 reverse. Os esfregaços sanguíneos também foram processados e examinados para a pesquisa de formas tripomastigotas do agente. DNA de T. evansi foi detectado em 18,52% (10/54) dos procionídeos, ocorrendo em mãos-peladas de cativeiro e quatis de vida livre e de cativeiro no estado do Pará. Não foram observadas formas tripomastigotas nos esfregaços sanguíneos. DNA de T. evansi foi detectado em P. cancrivorus e N. nasua no estado do Pará, sendo este o primeiro relato em P. cancrivorus.

Diagnóstico molecular e frequência de anticorpos anti-Leishmania infantum chagasi em cães do município de Belém, Pará / Molecular diagnosis and frequency of anti-Leishmania infantum chagasi antibodies in dogs in Belém/Pará, Brazil

Visceral leishmaniasis is a disease whose etiological agent in Brazil is Leishmania infantum chagasi. Dogs are considered urban reservoirs of the disease, being an indicator of the human cases occurrence. The present study aimed to diagnose L. infantum chagasi infection in stray and owned dogs in Belém, Pará State, by polymerase chain reaction (PCR) and indirect immunofluorescence assay (IFA) using two different antigens. [...] These animals were divided into two groups: stray dogs captured by the Center for Zoonosis Control (Group A) and owned dogs (Group B). Sera were analyzed by IFA testing for IgG using two different antigens: 1) Bio-Manguinhos/Fiocruz antigen kit (Ag-PRO) containing promastigotes of Leishmania sp. (Complex Major-Like), 2) Instituto Evandro Chagas Antigen (Ag-AMA) consisting of amastigotes of L. infantum chagasi. The evaluation of the two antigens was performed considering positive the reactions above the 1:80 dilution. Already PCR was performed with DNA isolated from whole blood of animals and amplified with the primers RV1 and RV2. Of the 335 samples analyzed, 10.4% (35/335) were positive by IFA (Ag-PRO) and 0.9% (3/335) with the Ag-AMA. The distribution of positive samples is given as follows: Group A 14.8% (25/169) with Ag-PRO and 1.2% (2/169) with Ag-AMA; Group B 6% (10/166) with Ag-PRO and 0.6% (1/166) with Ag-AMA, being that all samples positive by IFA with Ag-AMA also reacted with Ag-PRO, and none of the samples detected DNA of L. infantum chagasi. The findings of this study indicate that Belém can still be considered non-endemic area for canine visceral leishmaniasis and the nature of the antigen influences the result of the IFA for the detection of anti-L. infantum chagasi antibodies in dogs, and the IFA using promastigotes of Leishmania major-like antigen should be used with caution as a confirmatory diagnostic on epidemiological studies in non-endemic areas.

A leishmaniose visceral é uma enfermidade cujo agente etiológico no Brasil é o protozoário Leishmania infantum chagasi. Os cães são considerados reservatórios urbanos da doença, sendo indicadores da ocorrência de casos humanos. O presente trabalho teve como objetivo diagnosticar a infecção por L. infantum chagasi em cães domiciliados e errantes do município de Belém, estado do Pará, através da reação em cadeia da polimerase (PCR) e da reação de imunofluorescência indireta (RIFI), empregando dois antígenos distintos. [...] A avaliação dos dois antígenos foi realizada com as amostras reagentes a partir da titulação 1:80. Já a PCR foi realizada a partir do DNA extraído do sangue total dos animais e amplificado utilizando-se os iniciadores RV1e RV2. Das 335 amostras analisadas, 10,4% (35/335) foram reagentes na RIFI (Ag-PRO) e 0,9% (3/335) reagiram com o Ag-AMA. A distribuição das amostras positivas se deu da seguinte forma: Grupo A 14,8% (25/169) com Ag-PRO e 1,2% (2/169) com Ag-AMA; Grupo B 6% (10/166) com Ag-PRO e 0,6% (1/166) com Ag-AMA; sendo que todas as amostras positivas pelo teste de RIFI com o Ag-AMA também reagiram com o Ag-PRO e em nenhuma das amostras foi detectado o DNA de L. infantum chagasi. Os achados do presente estudo indicam que Belém ainda pode ser considerada área não endêmica para leishmaniose visceral canina e que a natureza do antígeno influencia no resultado da RIFI para a pesquisa de anticorpos anti-L. infantum chagasi em cães, sendo que a RIFI que utiliza formas promastigotas de Leishmania major-like como antígeno deve ser utilizada com cautela como método diagnóstico confirmatório em estudos epidemiológicos em áreas não endêmicas para LVC.

Diagnóstico molecular da infecção por hemoplasmas em gatos domésticos naturalmente infectados da cidade de Belém, Pará / Molecular diagnosis of haemoplasmas infection in naturally infected domestic cats from Belém, Pará

Mycoplasma haemofelis, 'Candidatus Mycoplasma haemominutum' e 'Candidatus Mycoplasma turicensis' são os agentes causadores da micoplasmose felina, que podem causar anemia aguda ou crônica. O objetivo deste trabalho foi determinar a ocorrência de hemoplasmas em gatos domésticos de Belém, Pará. Para isso, 201 gatos foram divididos em três grupos: Grupo A foi composto por 101 gatos de rua capturados pelo Centro de Controle de Zoonoses, o grupo B foi composto por 62 gatos domiciliados e saudáveis e o grupo C foi composto por 38 gatos domiciliados que apresentavam alguma afecção clínica. Foram coletadas amostras de sangue para a realização de Reação em Cadeia pela Polimerase (PCR) para detectar o DNA destes agentes, os quais foram sequenciados e alinhados. A análise estatística foi realizada para detectar a associação entre a infecção, o sexo dos animais e os grupos experimentais. O DNA de pelo menos uma das espécies de hemoplasmas pesquisados foi detectado em 19,9% (40/201) das amostras, sendo o DNA de 'Candidatus M. haemominutum' encontrado em 7,96% (16/201) das amostras, M. haemofelis em 1,49% (3/201) das amostras, enquanto que o DNA de 'Candidatus M. turicensis' foi detectado em 12,93% (26/201) das amostras. O DNA destes três agentes foi detectado em gatos dos grupos A e C, enquanto que no grupo B foi detectado apenas 'Candidatus M. turicensis' e 'Candidatus M. haemominutum' Foi detectada a influência do sexo sobre a infecção hemoplasmas apenas entre 'Candidatus M. haemominutum' e machos. Estes resultados mostraram que os hemoplasmas circulam entre os gatos domésticos em Belém e 'Candidatus M. turicensis' e 'Candidatus M. haemominutum' foram mais comuns do que M. haemofelis, especialmente em gatos vadios.

Mycoplasma haemofelis, 'Candidatus Mycoplasma haemominutum' and 'Candidatus Mycoplasma turicensis' are the causative agent of the feline mycoplasmosis, which could cause acute or chronic anemia. The aim of this work was to determine the occurrence of hemoplamas in domestic cats from Belém, Pará. To this, 201 cats were divided into three groups: Group A were composed by 101 stray cats captured by Zoonosis Control Center, group B were composed by 62 owners healthy cats and group C were composed by 38 owners cats that were suffering by some medical condition. Blood samples were collected to perform Polymerase Chain Reaction (PCR) to detect the DNA of these agents, which were sequenced and aligned. Statistical analysis was performed to detect association between the infection, the sex of the animals and experimental groups. The DNA of at least one of the hemoplasmas studied were detected in 19,9% (40/201) of the samples, being the DNA of 'Candidatus M. haemominutum' was found in 7.96% (16/201) of samples, M. haemofelis in 1.49% (3/201) of samples, while 'Candidatus M. turicensis' in 12.93% (26/201) of the samples. The DNA of these three agents was detected in cats from groups A and C, while in Group B was detected only 'Candidatus M. turicensis' and 'Candidatus M. haemominutum'. The influence of sex on hemoplasma infection was detected only between 'Candidatus M. haemominutum' and males. These findings showed that hemoplasma circulate among domestic cats in Belém, and 'Candidatus M. turicensis' and 'Candidatus M. haemominutum' were more common than M. haemofelis, especially in stray cats.

Infestation by Haematopinus quadripertusus on cattle in São Domingos do Capim, state of Pará, Brazil.

Severe infestation with lice was observed on crossbred cattle (Bos taurus indicus ×Bos taurus taurus) in the municipality of São Domingos do Capim, state of Pará, Brazil. Sixty-five animals were inspected and the lice were manually collected, preserved in 70% alcohol and taken to the Animal Parasitology Laboratory, School of Veterinary Medicine, Federal University of Pará, Brazil, for identification. The adult lice were identified as Haematopinus quadripertusus, and all the cattle examined were infested by at least one development stage of this ectoparasite. The specimens collected were located only on the tail in 80% (52/65) of the cattle, while they were around the eyes as well as on the ears and tail in 20% (13/65). Nits, nymphs and adults of the parasite were respectively collected from 98.46% (64/65), 38.46% (25/65) and 23.08% (15/65) of the animals examined. This is the first report of bovine pediculosis caused by H. quadripertusus in the state of Pará, Brazil. Further studies should be conducted to determine the occurrence pattern of this species in Brazil and its importance to livestock production.

Infestation by Haematopinus quadripertusus on cattle in São Domingos do Capim, state of Pará, Brazil / Infestação por Haematopinus quadripertusus em bovinos de São Domingos do Capim, Estado do Pará, Brasil

Severe infestation with lice was observed on crossbred cattle (Bos taurus indicus ×Bos taurus taurus) in the municipality of São Domingos do Capim, state of Pará, Brazil. Sixty-five animals were inspected and the lice were manually collected, preserved in 70% alcohol and taken to the Animal Parasitology Laboratory, School of Veterinary Medicine, Federal University of Pará, Brazil, for identification. The adult lice were identified as Haematopinus quadripertusus, and all the cattle examined were infested by at least one development stage of this ectoparasite. The specimens collected were located only on the tail in 80% (52/65) of the cattle, while they were around the eyes as well as on the ears and tail in 20% (13/65). Nits, nymphs and adults of the parasite were respectively collected from 98.46% (64/65), 38.46% (25/65) and 23.08% (15/65) of the animals examined. This is the first report of bovine pediculosis caused by H. quadripertusus in the state of Pará, Brazil. Further studies should be conducted to determine the occurrence pattern of this species in Brazil and its importance to livestock production.

Alta infestação por piolhos foi observada em vacas mestiças Bos taurus indicus e Bos taurus taurus do município de São Domingos do Capim, Estado do Pará, Brasil. Sessenta e cinco animais foram inspecionados e os piolhos foram coletados manualmente, armazenados em álcool 70% e transportados ao Laboratório de Parasitologia Animal da Faculdade de Medicina Veterinária da Universidade Federal do Pará para a identificação. Os exemplares adultos foram identificados como Haematopinus quadripertusus e todos os animais examinados apresentaram pelo menos um estágio de desenvolvimento do ectoparasito. Em 80% (52/65) dos animais, os exemplares coletados localizavam-se somente na cauda e em 20% (13/65) na região periocular, orelha e cauda. Lêndeas, ninfas e adultos foram coletados, respectivamente, em 98,46% (64/65), em 38,46% (25/65) e em 23,08% (15/65) dos animais examinados. Esse é o primeiro relato de pediculose bovina causada por H. quadripertusus no estado do Pará e mais estudos devem ser realizados para determinar a ocorrência dessa espécie no Brasil e a sua importância para a pecuária nacional.

First record of notoedric mange in ocelot (Leopardus pardalis Linnaeus, 1758) in the Amazon region, Brazil.

This paper describes a case of notoedric mange in an ocelot (Leopardus pardalis) in the Brazilian Amazon region. A young male of approximately four months of age that was illegally kept as a pet was apprehended in Altamira, State of Pará, northern Brazil. The animal was transported to the Mangal das Garças Park in the state's capital city of Belém. The ocelot had pruritus and lesions suggestive of scabies in the head. Skin scraping material was examined under optic microscopy. There was seen a large number of eggs, larvae, nymphs and adult mites. The mean female and male sizes were 230.2 × 200.4 µm and 137.6 × 104.9 µm. Based on the morphologic characteristics and morphometric analysis, this mite was classified as Notoedres cati. This is the first report of notoedric mange in L. pardalis from Brazilian Amazon.

First record of notoedric mange in ocelot (Leopardus pardalis Linnaeus, 1758) in the amazon region, Brazil / Primeiro relato de sarna notoédrica em jaguatirica (Leopardus pardalis Linnaeus, 1758) na região amazônica, Brasil

This paper describes a case of notoedric mange in an ocelot (Leopardus pardalis) in the Brazilian Amazon region. A young male of approximately four months of age that was illegally kept as a pet was apprehended in Altamira, State of Pará, northern Brazil. The animal was transported to the Mangal das Garças Park in the state's capital city of Belém. The ocelot had pruritus and lesions suggestive of scabies in the head. Skin scraping material was examined under optic microscopy. There was seen a large number of eggs, larvae, nymphs and adult mites. The mean female and male sizes were 230.2 × 200.4 µm and 137.6 × 104.9 µm. Based on the morphologic characteristics and morphometric analysis, this mite was classified as Notoedres cati. This is the first report of notoedric mange in L. pardalis from Brazilian Amazon.

O presente estudo descreve um caso de sarna notoédrica em uma jaguatirica (Leopardus pardalis) na região da Amazônia Brasileira. Um macho jovem com aproximadamente quatro meses de idade, ilegalmente mantido como animal de estimação, foi apreendido em uma residência em Altamira, Estado do Pará, Brasil. O animal foi transportado para o Parque Mangal das Garças, município de Belém, estado do Pará. A jaguatirica apresentava prurido e lesões sugestivas de escabiose na cabeça, por isso um raspado cutâneo foi realizado e examinado ao microscópio óptico. Foi observado um grande número de ovos, larvas, ninfas e ácaros adultos. Os exemplares fêmeas mediram em média 230,2 × 200,4 µm, e os exemplares machos mensuraram 137,6 × 104,9 µm. Com base nas características morfológicas e análises morfométricas, o ácaro foi classificado como Notoedres cati. Esse é o primeiro relato da sarna notoédrica em L. pardalis na Amazônia Brasileira.